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rabbit polyclonal anti grb2 (Proteintech)

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Proteintech rabbit polyclonal anti grb2
Rabbit Polyclonal Anti Grb2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+grb2/GRB2+Antibody/pm36898847-62-90-94
Average 93 stars, based on 28 article reviews

rabbit polyclonal anti grb2 - by Bioz Stars, 2026-10

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Article Title: In vivo CRISPR screens identify RhoV as a pro-metastasis factor of triple-negative breast cancer.
Article Snippet: The proteins were next splitted by SDS- PAGE gel and transferred on a polyvinylidene fluoride membrane (Roche). .. The membrane was blocked with 5% BSA in TBST solution for 60 min. Next, we utilized the primary antibody to blot at 4°C for 12– 16 h: rabbit polyclonal anti- HA (Cell Signaling Technology [CST]; 1:1000, no.3724), mouse polyclonal anti- GAPDH (ProteinTech; no.60004- 1- Ig, 1:5000), mouse polyclonal anti- Vinculin (ProteinTech; no.66305- 1- Ig, 1:5000), rabbit polyclonal anti- Flag (Sigma; no. F7425, 1:1000), rabbit polyclonal anti- PAK1 (CST; catalog no.2602, 1:1000), mouse polyclonal anti- β- Actin (CST; no.4970, 1:5000), rabbit polyclonal anti- His (ProteinTech; Cat No. 66005- 1- Ig, 1:1000), rabbit polyclonal anti- GRB2 (ProteinTech; Cat No. 10254- 2- AP, 1:1000), rabbit polyclonal anti- EGFR (CST; no.4267, 1:1000), and rabbit polyclonal anti- Phospho- EGFR (CST; no.4267, 1:1000). .. After washing with TBST three times, the membrane was incubated for 60 min at room temperature with HRP- conjugated goat anti- mouse antibody (Jackson ImmunoResearch; 1:5000) or goat anti- rabbit antibody (Jackson ImmunoResearch; 1:5000), and proteins were detected with chemiluminescence substrate (Thermo Fisher Scientific).

Article Title: In Vivo CRISPR Screens Identify RhoV as a Pro-Metastasis Factor of Triple-Negative Breast Cancer
Article Snippet: The proteins were next splitted by SDS-PAGE gel and transferred on a polyvinylidene uoride membrane (Roche). .. The membrane was blocked with 5% BSA in TBST solution for 60 minutes, next we utilized the primary antibody to blot at 4°C for 12–16 hours: rabbit polyclonal anti-HA (Cell Signaling Technology; 1:1,000, catalog no.3724), mouse polyclonal anti-GAPDH (ProteinTech; catalog no.60004-1-Ig, 1:5,000), mouse polyclonal anti-Vinculin (ProteinTech; catalog no.66305-1-Ig, 1:5,000), rabbit polyclonal anti-Flag (Sigma; catalog no. F7425, 1:1,000), rabbit polyclonal anti-PAK1 (Cell Signaling Technology; catalog no.2602, 1:1,000), mouse polyclonal anti-β-Actin (Cell Signaling Technology; catalog no.4970, 1:5,000), rabbit polyclonal anti-His (ProteinTech; Cat No. 66005-1- Ig, 1:1,000), rabbit polyclonal anti-GRB2 (ProteinTech; Cat No. 10254-2-AP, 1:1,000), rabbit polyclonal antiEGFR (Cell Signaling Technology; catalog no.4267, 1:1,000), rabbit polyclonal anti- Phospho-EGFR (Cell Signaling Technology; catalog no.4267, 1:1,000). .. After washing with TBST for three times, the membrane Page 6/23 was incubated for 60 minutes at room temperature with HRP-conjugated goat anti-mouse antibody (Jackson ImmunoResearch; 1:5,000) or goat anti-rabbit antibody (Jackson ImmunoResearch; 1:5,000), and proteins were detected with chemiluminescence substrate (Thermo Fisher Scienti c).

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